Tag

Part:BBa_K3755020

Designed by: Qi Xin   Group: iGEM21_ShanghaiTech_China   (2021-10-01)


6*His tag

Attached to the upstream or downstream of the protein, used to bind the protein to the medium in nickel ion affinity chromatography during the protein purification.


Usage and Biology

6*his tag is used as the purification tag attached to the target protein in protein purification. IIn nickel ion affinity chromatography, because the imidazole group of histidine residues has a strong affinity with the divalent nickel ion, the label together with the target protein can be adsorbed into the medium of the chromatographic column to achieve the effect of separation from impurities.

Experiment and Results

On pages of our composite part part:BBa_K3755011 and part:BBa_K3755012, protein purification experiments related to His tag and nickel ion affinity chromatography are described in detail.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None